![]() ![]() In the present study, we have tried to find out if automated (Immunoratio) and manual methods (H score and Allred score) produce comparable values and whether they can be used interchangeably. But so far, the results of automated ‘Immunoratio’ have not been compared with those of the manual methods. Shousha suggested a conversion table to make H-score and Allred score more equivalent. Whether the results of all the methods yield comparable values which can be used interchangeably is not yet clear. One well-known automated method is “Immunoratio”, which is freely available online as basic or advanced protocols. Some of the manual methods include H-score, immunoreactive Remmele score (IRS) and the most popular Allred score. Following immunohistochemical demonstration of the receptor expression, they can be quantitated by several well established methods, both manual and automated. Besides, American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) has recommended the use of image analysis for estimating the percentage of immunoreactive cells. The predictive and prognostic value of receptor expression may be refined and improved if the assessment of receptors is semi-quantitative. Absence of PR expression in an ER-positive cancer (Luminal B type), makes the lesion more aggressive with greater genetic instability and increased mutations. ![]() Its expression has a predictive value in assessing response to tamoxifen therapy in metastatic breast cancer. Significance of PR expression is more uncertain. Even without hormonal therapy, ER positive breast cancers have a better short-term prognosis than ER negative tumours. Introduction The expression of steroid hormone receptors – oestrogen receptor (ER) and progesterone receptor (PR) – by breast carcinoma is associated with strong positive correlation with the latter’s response to hormonal therapy and overall prognosis. There was a statistically significant positive correlation among all methods (p. Results: Both manual and automated methods produced results that were comparable. ![]()
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